产品说明
特异性
Bovine zonular fibrils. Reacts well with human elastin microfibrils and can be used to investigate the presence and organization of these fibrils either in Marfan patients or in fibroblasts obtained from these patients.
免疫原
Bovine zonular fibrils
应用
Research Category
Cell Structure
Immunoblot: antibody reacts with 350,000 Da protein released from bovine aortic smooth muscle cells, which is identical to that of fibrillin.
Immunohistochemistry: fresh frozen tissue: 1:200-1:500. Paraffin sections reactive after microwave citrate buffer antigen retrieval.
Immunoprecipitation: 5 microliters of antibody diluted in no more than 400 microliters of concentrated supernatants from bovine smooth muscle cells. We recommend either an anti-mouse IgG bead or a rabbit anti-mouse capture antibody followed by protein A.
ELISA
Optimal dilutions must be determined by the end user.
Research Sub Category
ECM Proteins
Anti-Fibrillin Antibody, clone 11C1.3 is an antibody against Fibrillin for use in ELISA, IF, IP, WB, IH(P).
外形
Liquid
Format: Purified
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 11C1.3, monoclonal |
species reactivity | bovine, human, pig |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunofluorescence: suitable immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | NM_000138.3 |
UniProt登记号 | P35555 |
运输 | dry ice |
Gene Information | human ... FBN1(2200) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich