Anti-Galectin-9 Antibody, clone 1G3

产品说明

一般描述

Galectin-9 (UniProt O00182; also known as Ecalectin, Gal-9, Tumor antigen HOM-HD-21, Urate transporter/channel protein) is encoded by the LGALS9 (also known as HUAT, LGALS9A) gene (Gene ID 3965) in human. The beta-galactoside-binding lectin, Galectin-9, possesses two distinct carbohydrate recognition domains (CRDs) linked together by a peptide domain of different length among the S, M, and L spliced isoforms. Galectin-9 plays a key role in a negative feed-back mechanism against Th1 immune response, where galectin-9 production from various cell types (e.g. fibroblasts and endothelial cells) is induced by interferon-gamma produced by CD4+ Th1 lymphocytes. The up-regulated galectin-9 in turn suppresses CD4+ Th1 lymphocytes, at least in part through stimulation of the Tim-3 receptor. The Tim-3 receptor on CD4+ Th1 cells from patients with multiple sclerosis (MS), rheumatoid arthritis, and auto-immune hepatitis is defective in its response to galectin-9. In addition, excessive galectin-9 production is reported in two human diseases associated with oncogenic viruses, nasopharyngeal carcinomas (NPC) associated with the Epstein-Barr virus (EBV) and chronic infection by the hepatitis C virus (HCV).

特异性

Clone 1G3 reacts with all three (L, M, and S) galectin-9 isoforms, but not galectin 1, 2, 3, 4, 7, 8, or 10.

免疫原

GST-tagged recombinant protein corresponding to human Galectin-9.

应用

This Anti-Galectin-9 Antibody, clone 1G3 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), ELISA and Immunocytochemistry for the detection of Galectin-9.
Immunohistochemistry Analysis: A representative lot detected galectin-9 immunoreactivity in hepatocytes as well as inflammatory leucocytes and Kupffer cells in paraffin-embedded liver sections from patients with hepatitis C or B infection, but not in non-infected liver specimens (Barjon, C., et al. (2012). Infect Agent Cancer. 7(1):16-26).
Immunohistochemistry Analysis: A representative lot detected galectin-9 immunoreactivity in malignant cells using various paraffin-embedded nasopharangeal carcinoma (NPC) tissue sections (Barjon, C., et al. (2012). Infect Agent Cancer. 7(1):16-26).
Western Blotting Analysis: A representative lot detected endogenous galectin-9 isoforms in HeLa, lymphoblastoid cell line (LCL) REMB1, and nasopharyngeal carcinoma (NPC) cell lines C15 & C666-1, as well as exogenously expressed galectin-9 isoforms in transfected HeLa cells, but not in Burkitt’s lymphoma (BL) BL2 cells (Barjon, C., et al. (2012). Infect Agent Cancer. 7(1):16-26).
ELISA Analysis: A representative lot selectively captured human & murine galectin-9 (mGal-9 M, hGAL-9 S & M isoforms), but not human galectin 1, 2, 3, 4, 7, 8, or 10 (Barjon, C., et al. (2012). Infect Agent Cancer. 7(1):16-26).
Immunocytochemistry Analysis: A representative lot detected galectin-9 immunoreactivity in formalin-fixed and paraffin-embedded lymphoblastoid cell line (LCL) REMB1, but not in Burkitt’s lymphoma (BL) BL2 cells (Barjon, C., et al. (2012). Infect Agent Cancer. 7(1):16-26).

质量

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Galectin-9 in 10 µg of HeLa nuclear extract.

目标描述

~38 kDa observed

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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