Anti-Girdin, coiled-coil region Antibody

产品说明

一般描述

Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.

特异性

Other Homologies: Mouse (97% sequence homology).

免疫原

GST-tagged recombinant protein corresponding to the coiled coil domain of rat Girdin.

应用

This Anti-Girdin, coiled-coil region Antibody is validated for use in ICC, Western Blotting, Immunoprecipitation for the detection of Girdin, coiled-coil region.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected Girdin in Cos-7 cells (Prof. P. Ghosh, Department of Medicine, Division of Gastroenterology and Hepatology, UCSD School of Medicine and Veterans Affairs Medical Center).

Western Blot Analysis: A representative lot detected Girdin in Cos-7 cell lysate. Uncharacterized bands may be observed in some cell lysates.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in HeLa cell lysates (Prof. P. Ghosh, Department of Medicine, Division of Gastroenterology and Hepatology, UCSD School of Medicine and Veterans Affairs Medical Center). Uncharacterized bands may be observed in some cell lysates.

质量

Immunocytochemistry Analysis: A 1:1000 dilution of this antibody detected Girdin in HeLa cells (Prof. A. Ghosh, Division of Biology, UCSD).

目标描述

~250 kDa observed. Some uncharacterized bands may be observed in some cell lysates.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa cells

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息