产品说明
一般描述
Monoclonal Anti-Glyoxalase I (rat IgG2b isotype) is derived from the hybridoma 6F10 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a mouse Glyoxalase I fusion protein. The glyoxalase system, which consists of glyoxalase I (GLO1), glyoxalase II, and a catalytic amount of reduced glutathione (GSH), is important part of cellular metabolism. GLO1 appears to be ubiquitously expressed in all mammalian cells, suggesting its biological importance.
免疫原
mouse Glyoxalase I fusion protein
应用
Monoclonal Anti-Glyoxalase I antibody produced in rat has been used in immunoblotting. and immunocytochemistry.
生化/生理作用
The glyoxalase system plays a major role to detoxify α-ketoaldehydes, especially methylglyoxal (MG), that are endogenously formed as a by-product of the triosephosphate isomerase reaction during glycolysis. A member of this system, GLO1 catalyzes the isomerization of a hemithioacetal, comprised of a nonenzymatic adduct of MG and glutathione (GSH), to the corresponding α-d-hydroxyacid thioester and S-D-lactoylglutathione. Studies have suggested that GLO1 may be important for brain function since, GLO1 in the brain is involved in Alzheimer′s disease, autism, anxiety and the regulation of theta oscillations during sleep.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
NACRES | NA.41 |
产品性质
生物来源 | rat |
偶联物 | unconjugated |
抗体形式 | purified from hybridoma cell culture |
antibody product type | primary antibodies |
克隆 | Clone 6F10, monoclonal |
形式 | buffered aqueous solution |
分子量 | ~21 kDa |
species reactivity | human, canine, mouse, rat, monkey |
包装 | antibody small pack of 25 μL |
浓度 | ~1.0 mg/mL |
technique(s) | immunocytochemistry western blot |
同位素/亚型 | IgG2b |
UniProt登记号 | Q04760 |
运输 | dry ice |
储存温度 | −20℃ |
Gene Information | human ... GLO1(2739) mouse ... Glo1(109801) rat ... Glo1(294320) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich