Anti-Glypican-1 Antibody, clone 4D1

产品说明

一般描述

Heparan sulfate proteoglycans (HSPGs) can be divided into general categories of
cell-associated forms, including syndecans, glypicans, and secreted extracellular matrix forms, such as perlecan. Glypicans contain six family members to date and are proteins bound to the outer surface of the plasma membrane by a glycosyl-phosphatidylinositol anchor and involved in cellular adhesion and migration and function as accessory molecules for signaling receptors, (Filmus, 2008 & Gui, 2006). Current publications indicate the main function of membrane-attached glypicans is to regulate the signaling of Wnts, Hedgehogs, fibroblast growth factors and bone morphogenetic proteins (BMPs), (Filmus, 2008).
Glypican-1 (GPC1) is over-expressed in human gliomas and appears to have a greater capability of promoting FGF-2 signaling in some gliomas versus HSPGs in normal astrocytes. Additional observations suggest that GPC1 can impact the growth of pancreatic cancer cells by attenuating tumor angiogenesis, (Whipple, 2008).

特异性

MAB2600 recognizes glypican-1.

免疫原

Full length recombinant protein.
Epitope: Full Length

应用

Immunohistochemistry:
A 1:50 dilution of a previous lot detected strong cytoplasmic staining of tumor cells in formalin-fixed, paraffin-embedded gangliobastoma tissue sample. There was negative staining of normal, uninvolved cells.
Research Category
Cell Structure
Anti-Glypican-1 Antibody, clone 4D1 detects level of Glypican-1 & has been published & validated for use in IH(P) & WB.
Research Sub Category
ECM Proteins

质量

Routinely evaluated by Western blot on human PC-12 cell lysate using 0.5 µg/mL of Anti-Glypican-1 at 1 mg/mL.

目标描述

Recognizes Glypican-1 at ~ 59 kDa

外形

Purified in 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% NaN3.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8ºC from date of receipt.

分析说明

Control
PC-12 cell lysate.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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