Anti-Helios Antibody, clone 22F6

产品说明

一般描述

Zinc finger protein Helios (Uniprot Q9UKS7; also known as Ikaros family zinc finger protein 2, Zinc finger DNA binding protein Helios, Zinc finger protein subfamily 1A member 2) is encoded by the IKZF2 (also known as ANF1A2, HELIOS, ZNF1A2, ZNFN1A2) gene (Gene ID 22807) in human. Helios, Ikaros, Aiolos, Eos, and Pegasus constitute a family of transcription factors characterized by six highly conserved C2H2 zinc fingers. Their four N-terminal zinc fingers mediate DNA binding, while the two C-terminal zinc fingers are responsible for homodimeric and heterodimeric protein interactions. Eos and Pegasus are expressed in a wide variety of tissues, whereas Helios, Ikaros, and Aiolos are limited to the hematopoietic system. Specifically, Helios expression is reported to be restricted to a subpopulation (approximately 70%) of Foxp3(+) T cells in mice and humans.

特异性

Expected to react with isoform 1, 2 (Helios 1v), 3 (Helios del(Ex3,4)), 4 (Helios del(Ex6)v), 5 (Helios 1+2a,2b, Helios 1v+2a, Helios del(Ex3)+2a), 6 (Helios 1+5a, Helios 1+5a,5b), 7 (Helios del(Ex5)v), but not isoform 8 (Helios S).

免疫原

Linear peptide corresponding to a human Helios N-terminal sequence.
Epitope: N-terminal

应用

Research Sub Category
Immunoglobulins & Immunology
This Anti-Helios Antibody, clone 22F6 is validated for use in Flow Cytometry for the detection of Helios.
Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected Helios in 1X10E6 human Jurkat cells.
Flow Cytometry Analysis: A representative lot detected Helios immunoreactivity among the majority of CXCR5-positive cells within the CD4+Foxp3+ population of murine peripheral lymph node (LN) cells (Chung, Y., et al. (2011). Nat Med.17(8):983-988).
Flow Cytometry Analysis: A representative lot detected Helios immunoreactivity in the Foxp3-positive, but not Foxp3-negative, subpopulation of CD4+ cells from murine peripheral lymph node (LN) and human PBMCs (Thornton, A.M., et al. (2010). J Immunol.184(7):3433-3441).
Flow Cytometry Analysis: A representative lot revealed the age dependency of Helios-positive population among CD4+ murine splenocytes and CD4+/CD8- murine thymocytes (Thornton, A.M., et al. (2010). J Immunol.184(7):3433-3441).
Flow Cytometry Analysis: A representative lot detected increased Helios-positive population among the TGF-beta-stimulated Treg, but not Teff cells from murine peripheral lymph node (LN) and human PBMCs (Thornton, A.M., et al. (2010). J Immunol.184(7):3433-3441).

Research Category
Inflammation & Immunology

质量

Evaluated by Flow Cytometry in human PBMCs.

Flow Cytometry Analysis: 1.0 µg of this antibody detected Helios in human PBMCs.

目标描述

~ 59 kDa calculated

外形

Format: Purified
Purified Armenian hamster monoclonal IgG antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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