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Anti-HGPRT Antibody, clone 13H11.1

品牌
Sigma-Aldrich
货号
MABS528
规格纯度
clone 13H11.1, from mouse
参考价格
5352.52 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-HGPRT Antibody, clone 13H11.1
Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase
产品介绍:

产品说明

一般描述

Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8; UniProt P00492; also known as HGPRT, HGPRTase) is encoded by the HPRT1 (also known as HGPRT, HPRT) gene (Gene ID 3251) in human. HPRT is a purine salvage enzyme that catalyzes the condensation of 5-phosphoribosyl 1-pyrophosphate (PRPP) and hypoxanthine or guanine to form inosine 5-monophosphate (IMP) and guanosine 5-monophosphate (GMP), respectively. PRPP is an essential compound for the de novo pathway of purine synthesis as well as the synthesis of pyrimidine compounds. PRPP concentrations in erythrocyte are markedly increased in individuals with HPRT deficiency. Complete HPRT deficiency leads to Lesch–Nyhan disease (LND) characterized by uric acid overproduction, motor dysfunction, cognitive disability, and self-injurious behavior. Partial deficiency of HPRT activity causes Lesch–Nyhan variant (LNV) characterized by hyperuricemia and variable degree of neurological manifestations, depending on the residual enzyme activity.

免疫原

Epitope: Internal
GST-tagged human HGPRT recombinant fragment lacking N- and C-terminal regions.

应用

This Anti-HGPRT, clone 13H11.1 is validated for use in Western Blotting for the detection of HGPRT.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HGPRT in 10 µg of HeLa cell lysate.

目标描述

~25 kDa observed

外形

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆13H11.1, monoclonal
species reactivityhuman
technique(s)western blot: suitable
同位素/亚型IgG2bκ
NCBI登记号NP_000185
UniProt登记号P00492
运输wet ice
Gene Informationhuman ... HPRT1(3251)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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