产品说明
一般描述
Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
特异性
Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.
免疫原
KLH-EDC-conjugated linear peptide corresponding to the unknown of Human Oct-4.
Epitope: unknown
应用
Research Category
Stem Cell Research
Stem Cell Research
Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected human Oct-4 in 10 µg of H9 human ES cells.
This Anti-Human Oct-4 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Human Oct-4.
Research Sub Category
Neural Stem Cells
Developmental Signaling
质量
Evaluated by Western Blotting in H9 human ES cell lysate and mouse ES cells lysate.
Western Blotting Analysis: A 0.25 µg/mL dilution of this antibody detected human Oct-4 in 10 µg of H9 human ES cell lysate but not in 10 µg of mouse ES cells lysate.
目标描述
~40 kDa observed
外形
Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Antigen Affinity Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.75 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human |
species reactivity (predicted by homology) | chimpanzee (based on 100% sequence homology), goat (based on 100% sequence homology) |
technique(s) | immunocytochemistry: suitable western blot: suitable |
NCBI登记号 | NP_002692 |
UniProt登记号 | Q01860 |
运输 | wet ice |
Gene Information | human ... POU5F1(5460) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich