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Anti-Integrin α5β1 Antibody, clone BMB5

Anti-Integrin α5β1 Antibody, clone BMB5

品牌
Sigma-Aldrich
货号
MAB2514
规格纯度
ascites fluid, clone BMB5, Chemicon®
参考价格
6190.47 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Anti-Integrin α5β1 Antibody, clone BMB5
VLA-5
产品介绍:

产品说明

特异性

Antibody MAB2514 was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.Specificity of the antibody was verified by its ability to immunoprecipitate alpha5beta1 heterodimers from (125)-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions.

免疫原

The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.

应用

Research Category
Cell Structure
Research Sub Category
Integrins
Inhibition of cell adhesion to fibronectin: 10 μg/mL

Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMB5 alone may not be complete.

Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μg

Flow cytometry: 10μg/mL

Western blot (non-denaturing gel)

Optimal working dilutions must be determined by end user.
This Anti-Integrin α5β1 Antibody, clone BMB5 is validated for use in FC, IP, WB, IH, FUNC for the detection of Integrin α5β1.

外形

Liquid, containing no preservatives.

储存及稳定性

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源rat
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆BMB5, monoclonal
species reactivitymouse
manufacturer/tradenameChemicon®
technique(s)flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG3
NCBI登记号NM_002205.2
UniProt登记号P08648
运输dry ice
Gene Informationhuman ... ITGA5(3678)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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