Anti-Integrin α6 Antibody, clone BnC6B4

产品说明

特异性

Reactive with chicken integrin alpha6, as demonstrated by experiments with cDNA-transfected NIH3T3 cells followed by immunoblots.

免疫原

Integrin alpha subunits purified from adult chicken brain tissue, denatured in 6M guanidine isothiocyanate, and depleted of beta1 subunit.

应用

Anti-Integrin α6 Antibody, clone BnC6B4 is an antibody against Integrin α6 for use in IP, WB, IC, IH.
Western blot

Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor.

Immunohistochemistry: Effective for immunohistochemistry of methanol-fixed cryosections. An overnight methanol fix is suggested. Previous experiments have used 14 micron sections of chicken embryonic tissue. Typical antibodies dilutions are 35 μg/mL, with overnight antibody incubation, followed by detection with a goat anti-mouse Ig -FITC secondary antiobody.

Immunocytochemistry: Effective for methanol-fixed cultured cells (15 minute fixation).

Immunoblot: Recognizes a 130 kDa band in chicken brain tissue and a 140 kDa band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation).

Optimal working dilutions must be determined by end user.

外形

Format: Purified

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Manufactured by Daiichi Fine Chemical Co., Ltd

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