Anti-Integrin β1D Antibody, CT cytoplasmic domain, clone 2B1

产品说明

一般描述

Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated alpha and beta subunits. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. There are two major forms of integrin beta 1:beta 1A and beta 1D which differ in 13 amino acids. Their distribution pattern in adult tissues is mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle, which instead express the beta1D variant.

特异性

MAB1900 is specific for the cytoplasmic domain of integrin beta1D subunit. Integrin beta1D is present in cardiac and skeletal muscle. In proliferating C2C12 cells, beta1D is not expressed; C2C12 after differentiation into myotubes, however, express high levels of beta1D integrin. beta1D integrin was also found expressed in adult mouse extracts from heat, hind limb muscle, and tongue, with a faint band in skin. No reactivity was observed in extracts from lung, kidney, liver, or spleen {van der Flier et al, 1997}.

免疫原

Synthetic peptide including an appending N-terminal cysteine: CQENPIYKSPINNFKNPNYGRKAGL coupled to KLH.
Epitope: C-terminus cytoplasmic domain

应用

Research Category
Cell Structure
Research Sub Category
Integrins
This Anti-Integrin β1D Antibody, C-terminus cytoplasmic domain, clone 2B1 is validated for use in WB, IC, IH for the detection of Integrin β1D.
Western Blotting: 1:100-1:1000; recognizes a 116kDa band in reduced, 7% PAGE-SDS westerns in differentiated C2C12 cells and some adult tissues {See van der Flier, 1997}. 1% SDS lysis buffer is recommended over RIPA or traditional reducing sample buffer.

Immunocytochemistry:

Immunohistochemistry: 1:25-1:200. Frozen, unfixed tissue sections, post-fixed with 2% PFA after primary and secondary antibody additions {van der Flier, 1997}. Alternatively, 4-7μm cyrosections fixed with cold acetone for 2 minutes and stored at -80°C until used. Airdried sections are rehydrated in PBS prior to use; detection via ABC systems is encouraged.

Optimal working dilutions must be determined by the end user.

外形

Format: Purified
Purified immunoglobulin. Liquid in PBS containing 0.09% sodium azide.

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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