您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Anti-Interleukin-8 Antibody, clone 8M6

Anti-Interleukin-8 Antibody, clone 8M6

品牌
Sigma-Aldrich
货号
MAB1044
规格纯度
clone 8M6, Chemicon®, from mouse
参考价格
5211.96 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Interleukin-8 Antibody, clone 8M6
IL-8
产品介绍:

产品说明

特异性

Reacts with ovine IL-8. No reactivity detected to recombinant ovine IL-1beta, IL-6, MCP-1 or TNFalpha.

免疫原

Recombinant ovine IL-8

应用

Research Sub Category
Cytokines & Cytokine Receptors
This Anti-Interleukin-8 Antibody, clone 8M6 is validated for use in ELISA, FC, NEUT, WB for the detection of Interleukin-8.
Capture antibody in ELISA. Recommended coating concentration 5 μg/mL. See suggested protocol.

Flow cytometry: use 1 μg/test

Neutralizes recombinant ovine IL-8.

Western blotting

Optimal working dilutions must be determined by the end user.

Suggested ELISA Protocol For Ovine IL-8

1. Coat high capacity microtitre wells (e.g. Nunc maxisorp) with monoclonal anti-IL-8 antibody (Chemicon Catalog Number MAB1044) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4ºC.

2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST).

3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST.

4. Dilute rabbit anti-IL-8 (Chemicon Catalog Number AB1840) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST.

5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST.

6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulfuric acid.

Notes:

The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates.

Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents.

Assay sensitivity may be altered by adjusting incubation times and reagent concentrations.
Research Category
Inflammation & Immunology

外形

Liquid in PBS containing 0.1% sodium azide.
Format: Purified

储存及稳定性

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆8M6, monoclonal
species reactivitybovine, sheep, goat
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
flow cytometry: suitable
neutralization: suitable
western blot: suitable
同位素/亚型IgG2a
NCBI登记号NM_000584.2
UniProt登记号P10145
运输dry ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品