产品说明
一般描述
Inversin is a tubulin-associated protein, which is highly expressed in the cilia of renal tubules. It contains sixteen ankyrin repeats at the N-terminus and two IQ domains at the C-terminus. The ankyrin repeats facilitate protein-protein interactions, whereas the IQ domains are involved in calmodulin binding. Previous studies have indicated that inversin modulates the Wnt signaling pathway, and interacts with a plethora of intracellular signaling targets, including Disheveled, Frizzled-8, alpha- and beta catenins, NPHP1, and NPHP3. Inversin is localized to mitotic spindle fibers, and plays an important role in renal development and left-right axis determination. Defects in inversin result in nephronophthisis type 2, an autosomal recessive cystic kidney disease.
特异性
No homology to Rat was found.
免疫原
KLH-conjugated linear peptide corresponding to human Inversin.
应用
Research Category
Cell Structure
Anti-Inversin Antibody detects level of Inversin & has been published & validated for use in Western Blotting & IHC.
Western Blot Analysis: 1 µg/mL from a representative lot detected Inversin in 10 µg of human liver tissue lysate.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Inversin in human kidney tissue.
Research Sub Category
Cytoskeleton
质量
Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Inversin in 10 µg of HEK293 cell lysate.
目标描述
~165/12 kDa observed. Uniprot describes 3 isoforms at ~11, ~100, and ~118 kDa The calculated molecular weights are 11 and 118 kDa, however Inversin has been shown as a ~12 and ~165 kDa band in western blots (Nurnberger, J., et al. (2004). J Am Soc Nephrol. 15:1700–1710.). Uncharacterized bands may appear at ~45, ~53, and ~55 kDa
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HEK293 cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human |
species reactivity (predicted by homology) | rhesus macaque (based on 100% sequence homology) |
technique(s) | immunohistochemistry: suitable western blot: suitable |
NCBI登记号 | NP_055240 |
UniProt登记号 | Q9Y283 |
运输 | wet ice |
Gene Information | human ... INVS(27130) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich