Anti-Kinectin Antibody

产品说明

一般描述

Organelle transport is facilitated by molecular motors, such as kinesin and dynein, which interact with microtubules to transport cargo in an ATP-dependent manner. The shuttling of organelles is also dependent on kinectin, a transmembrane ER protein that associates with kinesin and stimulates its ATPase activity. Kinesin-kinectin dynamics have also been linked to focal adhesion growth. In addition, kinectin has been shown to activate the RhoG GTPase pathway which involves Rac1 and Cdc42 signaling. Kinectin is expressed in testis, ovary, spleen, thymus, prostate, colon, and small intestine.

特异性

This antibody recognizes the lumenal region of Kinectin.
Other homologies: Mouse (82% Mouse sequence homology) and Rat (79% sequence homology).

免疫原

Epitope: Lumenal region
KLH-conjugated linear peptide corresponding to the lumenal region of human Kinectin.

应用

Research Category
Cell Structure
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Kinectin in HeLa and A431 cells.
Detect Kinectin using this Anti-Kinectin Antibody validated for use in Western Blotting, ICC.
Research Sub Category
Cytoskeleton

质量

Evaluated by Western Blot in human testis tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Kinectin in 10 µg of human testis tissue lysate.

目标描述

~158 kDa observed. Uniprot describes 3 isoforms at ~149, ~150, and ~156 kDa

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Human testis tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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