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位置:首页 > 品牌 > Sigma-Aldrich > Anti-Lamin A/C Antibody, clone 2E8.2 Antibody, Alexa Fluor® 488 Conjugate

Anti-Lamin A/C Antibody, clone 2E8.2 Antibody, Alexa Fluor® 488 Conjugate

品牌
Sigma-Aldrich
货号
MABT538-AF488
规格纯度
clone 2E8.2, from mouse, ALEXA FLUOR® 488
参考价格
5093.23 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Anti-Lamin A/C Antibody, clone 2E8.2 Antibody, Alexa Fluor® 488 Conjugate
70 kDa lamin, Renal carcinoma antigen NY-REN-32
产品介绍:

产品说明

一般描述

Lamin-A/C (UniProt: P02545; also known as 70 kDa lamin, Renal carcinoma antigen NY-REN-32) is encoded by the LMNA (also known as LMN1) gene (gene ID: 4000) in human. Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Proteolytic cleavage of 18 residues from the C-terminal of prelamin-A/C results in the production of lamin-A/C. Proteolytic cleavage requires prior farnesylation and methylation, and absence of these blocks cleavage. The prelamin-A/C maturation pathway includes farnesylation of CAAX motif, ZMPSTE24/FACE1 mediated cleavage of the last three amino acids, methylation of the C-terminal cysteine and endoproteolytic removal of the last 15 C-terminal amino acids. Farnesylation of prelamin-A/C also facilitates nuclear envelope targeting. Lamin A and C are present in equal amounts in the lamina of mammals. Lamin plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin C differs from canonical form A in the following manner: amino acids 567-572 are changed from GSHCSS to VSGSRR and form C does not amino acids 573-664 seen in canonical form A.

特异性

Clone clone 2E8.2 specifically recognizes Lamin A/C in HeLa cells. This antibody has been shown to bind to an epitope between amino acids 464-572 in the C-terminal half.

免疫原

GST/His-tagged recombinant fragment corresponding to 109 amino acids from the C-terminal half of human Lamin A/C.

应用

Research Category
Cell Structure
This mouse monoclonal Anti-Lamin A/C Antibody, clone 2E8.2, Alexa Fluor® 488 Conjugate, Cat. No. MABT538-AF488 is used for the detection of Lamin A/C by Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Lamin A/C in A431 cells.

The unconjugated antibody (Cat. No. MABT538) is shown to be suitable also for Flow Cytometry, Immunohistochemistry, and Immunoprecipitation applications.

质量

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Lamin A/C in HeLa cells.

目标描述

74.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified mouse monoclonal antibody in PBS with 1.5% BSA and 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

法律信息

ALEXA FLUOR is a registered trademark of Life Technologies

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源mouse
偶联物ALEXA FLUOR® 488
抗体形式purified antibody
antibody product typeprimary antibodies
克隆2E8.2, monoclonal
species reactivityhuman
technique(s)immunocytochemistry: suitable
同位素/亚型IgG1κ
NCBI登记号NP_733821
UniProt登记号P02545
运输ambient
Gene Informationhuman ... LMNA(4000)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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