Anti-Linear Ubiquitin Antibody, clone LUB9

产品说明

一般描述

Ubiquitin functions as a marker to indicate the fate of other proteins in the cell. It can be attached as a single molecule or as a polyubiquitin chain. Although ubiquitinated proteins are oftentimes tagged for degradation by the 20S proteosome, ubiquitination can also indicate alternative fates depending on the lysine residue that is involved in linking the ubiquitin chain to the target protein. Ubiquitin chains linked by Lys33 to kinase substrates such as AMP-activated protein kinases, (AMPKα1, BRSK1, MARK3, MARK4, and NUAK1), may activate or deactivate these proteins, supporting the role for uniquitin in regulating signaling.

特异性

This antibody detects only Linear Ubiquitin.

免疫原

Ovalbumin-conjugated linear peptide corresponding to human Linear Ubiquitin.

应用

Anti-Linear Ubiquitin Antibody, clone LUB9 | MABS451 is an antibody against Linear Ubiquitin Antibody for use in Western Blotting.
Western Blotting Analysis: A representative lot detected Linear Ubiquitin in (M1-linked) ubiquitin chains (Yoshiteru, S. et al. (2013). EMBO. 32:2463-2476).
Research Category
Signaling
Research Sub Category
Signaling Neuroscience

质量

Evaluated by Western Blotting in recombinant protein Linear Polyubiquitin chains, K48 Ub 2-7, K63 Ub 2-7, and K11-only Ubn-ubiquitinylated HM substrate protein.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected 10 µg of Linear Polyubiquitin recombinant protein chains, bands were not detected in K48 Ub 2-7, K63 Ub 2-7, and K11-only Ubn-ubiquitinylated HM substrate protein.

目标描述

Multiple bands observed

外形

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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