Anti-LRP6, clone A59, Ectodomain Antibody

产品说明

一般描述

Low density lipoprotein receptor-related protein 6, also known as LRP6, is a member of the low density Lipoprotein receptor gene family (LDLR). LDLR are cell surface proteins. LRP6 plays a crucial role in embryonic development by functioning as a co-receptor for the Wnt signaling pathway. LRP6 is highly homologous and presents itself as a dimer in the cell membrane until it is phosphlorylated by Wnt. LRP6 has a high affinity with the Dickkopf (DKK) family of antagonists which, when bound, block Wnt. When LRP6 binds to Wnt it is phosphlorated at multiple sites, which results in Axin, a scaffolding protein, being recruited to the cell membrane and β-catenin activation. β-catenin prevents Wnt and LRP6 from binding. LRP6 is associated with autosomal dominant coronary artery disease type 2. Mutations resulting in the omission of the extracellular domain of LPR6 results in constant activity whereas carboxy intracellular domain exclusion has a negative effect, resulting in the blocking of Wnt signaling.

免疫原

Epitope: Ectodomain Domain
Purified protein corresponding to the ectodomain domain of human LRP6.

应用

This Anti-LRP6 antibody, clone A59, Ectodomain is validated for use in flow cytometry, IP & ICC for the detection of LRP6.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling
Flow Cytometry Analysis: A representative lot from an independent laboratory detected LRP6, Ectodomain in Chimeric LRP6 expressing 293T cells (Yasui, N., et al. (2010). J Immunol Methods. 352(1-2):153-160.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated LRP6, Ectodomain from Wnt3a-treated HEK293T cell lysate (Yasui, N., et al. (2010). J Immunol Methods. 352(1-2):153-160.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected LRP6, Ectodomain in HeLa S3 cells (Yasui, N., et al. (2010). J Immunol Methods. 352(1-2):153-160.).

质量

Evaluated by Flow Cytometry in HEK293 cells.

Flow Cytometry Analysis: 2 µg of this antibody detected LRP6, Ectodomain in 1X10E6 HEK293 cells.

目标描述

180 kDa calculated

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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