Anti-Lysine-specific demethylase 7 Antibody

产品说明

一般描述

Lysine-specific demethylase 7, also known as JmjC domain-containing histone demethylation protein 1D, and encoded by the gene JHDM1D/KDM7/KIAA1718, is an important histone demethylase particularly for brain development. JHDM1D specifically demethylates dimethylated ′Lys-9′ and ′Lys-27′ (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 ′Lys-20′ residue (H4K20Me1), thereby playing a central role in histone code. JHDM1D also specifically binds trimethylated ′Lys-4′ of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. JHDM1D also demethylates H3K9me2 in absence of H3K4me3 and JHDM1D has activity toward H4K20Me1 only when the nucleosome is used as a substrate and when not the histone octamer is used as substrate. JHDM1D is located in the nucleus and widely expressed but lowered expression is observed in glial cells and lymphoid cells. JHDM1D demonstrates tumor suppression by regulating angiogenesis factors when overexpression under starvation conditions in certain cancers, thus JHDM1D is being examined as a potential anti-cancer target.

特异性

This antibody is expected to cross react with two known isoforms of Lysine-specific demethylase 7 based upon immunogen design.

免疫原

KLH-conjugated linear peptide corresponding to human Lysine-specific demethylase 7.

应用

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Detect Lysine-specific demethylase 7 using this rabbit polyclonal antibody, Anti-Lysine-specific demethylase 7 Antibody validated for use in western blotting.

质量

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Lysine-specific demethylase 7 in 10 µg of HEK293 cell lysate.

目标描述

~110 kDa observed. Uniprot describes two isoforms at 107 kDa (isoform 1) and 92 kDa (isoform 2).

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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