Anti-MMP-2 Proform Antibody, NT, clone CA-4001

产品说明

特异性

It recognizes a protein of 72 kDa which is identified as pro (latent) form (active form is 62 kDa) of matrix metalloproteinase-2 and any fragment with N-terminal (proform) domain still attached. Antibody shows no cross-reaction with pro and active forms of MMP-9. MMP-2 is synthesized as 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids. MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). Cellular Localization: Cytoplasmic.

免疫原

N-terminal peptide APSPIIKFPGDVAPKTDK of procollagenase IV, coupled to KLH.{Marqulies, et al 1992}

应用

Western Blot (0.5-1.0 μg/mL for 2 hours at room temperature, non-reduced conditions. However, chicken samples react best when fully reduced. Antibody dilution buffers should be simple for best results, PBS or TBS with 0.05% tween only. Immunogen used is highly conserved between species, thus immunoabsorption to pro-MMP2 in milk may impair reactivity.

Immunohistochemistry on frozen and formalin-fixed, paraffin-embedded tissue sections: 2-10 μg/mL for 30 minutes at room temperature. {Seftor, et al 2001} and Gottsch ML et al 2002}. Antigen retrieval is optional in most cases. Antibody dilution solutions should not contain serum because of possible immunoabsorption of the antibody to proMMP2 present in the solution.

Blocking: Inhibition of MMP-2 Activity (2-4 μg/mL).



Optimal working dilutions must be determined by end user.
Anti-MMP-2 Proform Antibody, N-terminus, clone CA-4001 detects level of MMP-2 Proform & has been published & validated for use in WB, IH(P).

联系

Replaces: 04-1048

外形

Purified from ascites fluid by Protein G chromatography. Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide
Format: Purified

分析说明

Control
POSITIVE CONTROL: HFL-1 cells. Conditioned, serum-free medium from (TPA-treated) human fetal lung cells. Placenta.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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