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Anti-Neph1 Antibody, cytoplasmic domain

品牌
Sigma-Aldrich
货号
ABS1511
规格纯度
from rabbit, purified by affinity chromatography
参考价格
5016.79 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Neph1 Antibody, cytoplasmic domain
Kin of IRRE-like protein 1, Kin of irregular chiasm-like protein 1, Nephrin-like protein 1, Neph1, cytoplasmic domain
产品介绍:

产品说明

一般描述

Kin of IRRE-like protein 1 (UniProt Q80W68; also known as Nephrin-like protein 1, Kin of IRRE like 1, Kin of irregular chiasm-like protein 1) is encoded by the Kirrel (also known as 6720469N11Rik, Kirrel1, Neph1) gene (Gene ID 170643) in murine species. Podocytes are specialized epithelial cells that are critical components of the glomerular filtration barrier. Glomerular injury, such as that caused by renal ischemia, is often characterized by the effacement of podocytes, loss of slit diaphragms, and proteinuria. The podocyte proteins Neph1 and nephrin are critical for maintaining the structural integrity of slit diaphragm and therefore the filtration function of the glomerulus by organizing a signaling complex at the podocyte cell membrane. Cytoplasmic domain of Neph1 plays a key role in actin cytoskeleton remodeling events that are directly associated with Fyn-mediated Neph1 pTyr637/638 phosphorylation that leads to the recruitment of adapter proteins and signaling molecules, such as Grb2, Csk tyrosine kinase, and ZO-1. Phosphorylation-induced association between Neph1 and ZO-1 plays a key role in defining the tight junction formation in podocytes.

特异性

Expected to react with murine spliced isoforms 1&2, as well as all three human spliced isoforms.

免疫原

Epitope: cytoplasmic domain
His-tagged recombinant protein corresponding to the cytoplasmic domain of mouse Neph1.

应用

Western Blotting Analysis: A representative lot detected endogenous rat glomeruli Neph1 and exogenously expressed mouse Neph1 in COS-7 cells (Barletta, G.M., et al. (2003). J Biol Chem. 278(21):19266-19271).
Western Blotting Analysis: A representative lot detected ischemia-induced Neph1 membrane-to-cytosol translocation in human podocytes (Wagner, M.C., et al. (2008). J Biol Chem. 283(51):35579-35589).
Western Blotting Analysis: A representative lot detected Neph1 in mouse glomeruli & cultured human podocytes (Arif, E., et al. (2011). Mol Cell Biol. 31(10):2134-2150).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Neph1 from rat glomerular and human podocyte cell lysates (Arif, E., et al. (2011). Mol Cell Biol. 31(10):2134-2150).
Immunofluorescence Analysis: A representative lot detected Neph1 using both paraffin-embedded and frozen rat kidney sections (Arif, E., et al. (2011). Mol Cell Biol. 31(10):2134-2150; Barletta, G.M., et al. (2003). J Biol Chem. 278(21):19266-19271).
Electron Microscopy Analysis: A representative lot detected Neph1 in frozen rat kidney sections (Barletta, G.M., et al. (2003). J Biol Chem. 278(21):19266-19271).
Immunocytochemistry Analysis: A representative lot detected Neph1 in cultured human podocytes (Arif, E., et al. (2014). J Biol Chem. 289(14):9502-9518; Arif, E., et al. (2011). Mol Cell Biol. 31(10):2134-2150; Wagner, M.C., et al. (2008). J Biol Chem. 283(51):35579-35589).
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Detect Neph1 using this rabbit Polyclonal Anti-Neph1 Antibody, cytoplasmic domain, Cat. No. ABS1511, validated for use in Western Blotting, Immunoprecipitation, Immunofluorescence, Electron Microscopy and Immunocytochemistry.

质量

Evaluated by Western Blotting in rat kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Neph1 in rat kidney tissue lysate.

目标描述

~110 kDa observed. Target band size appears larger than the calculated molecular weight (87.2 kDa/murine isoform 1, 70.0 kDa/murine isoform 2, 72.4-85.0 kDa/human isoform 1-3) due to posttranslational modifications (glycosylation & phosphorylation). An uncharacterized band ~25 kDa can also be seen in some samples.

外形

Purified rabbit polyclonal antibody in buffer containing PBS/glycine/5% BSA, .02% Sodium Azide and 30% glycerol.
Affinity purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityrat, human, mouse
technique(s)electron microscopy: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号NP_001164456
UniProt登记号Q80W68
运输wet ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 3

Sigma-Aldrich

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