Anti-Nephrin Antibody

产品说明

一般描述

Nephrin (NPHS1, NPHN), also known as is Renal glomerulus-specific cell adhesion receptor, is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.

免疫原

KLH-conjugated linear peptide corresponding to human Nephrin.

应用

Detect Nephrin using this rabbit polyclonal antibody, Anti-Nephrin Antibody validated for use in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 1 µg/mL from a representative lot detected Nephrin in mouse kidney tissue.

Immunofluorescence Analysis: 10 µg/mL from a representative lot detected Nephrin in mouse kidney tissue.

质量

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Nephrin in 15 µg of mouse kidney tissue lysate.

目标描述

~100 kDa observed. Uncharacterized bands may appear in some lysate(s). Despite its calculated molecular weight of 135 kDa, Nephrin often migrates at a lower than expected size in SDS-PAGE.

外形

Antigen Affinity Purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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