产品说明
一般描述
Anti-Neurofilament 160 antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the NN18 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with neurofilaments purified from pig spinal cord. Neurofilaments (NFs) belong to the intermediate filament (IF) family and are expressed mainly in cells or tissues of neuronal origin. Three major NFs subunits were discovered: light (NF-L, ~68 kDa), medium (NF-M, ~160 kDa) and heavy (NF-H, ~200 kDa), which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 160, also known as NF-M or Neurofilament triplet M protein.
免疫原
neurofilaments purified from pig spinal cord
应用
Anti-Neurofilament 160 antibody has been used in immunoblotting, Immunohistochemistry, Immunofluorescence
生化/生理作用
NFs can accumulate in large numbers within cell bodies and proximal axons of affected neurons in a variety of pathologies, including Charcot-Marie-Tooth disease (CMT), neurofilament inclusion disease (NFID), giant axonal neuropathy (GAN), diabetic neuropathy, spinal muscular atrophy (SMA) and spastic paraplegia. In addition, NFs accumulation were detected in Alzheimer′s (AD) and Parkinson′s disease (PD) patients. Elevated concentrations of Neurofilament 160 have been shown in cerebrospinal fluid (CSF) and serum samples from patients with brain injury.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | NN18, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~160 kDa |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunoblotting: 0.3-0.6 μg/mL using rat brain extract immunofluorescence: 10-20 μg/mL using human bone marrow neuroblast SHSY5Y cell line immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human brain sections |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P07197 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
