产品说明
一般描述
Rabbit polyclonal antibody. Recognizes the ~65 kDa NF-κB p65 (RelA) protein.
This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2).
Recognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells.
免疫原
a synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH
Human
包装
100 μL in Plastic ampoule
应用
ELISA (see comments)
Gel Shift (see comments)
Immunoblotting (1:500, chemiluminescence)
Immunoprecipitation (see comments)
警告
Toxicity: Standard Handling (A)
外形
In 5 mM sodium phosphate buffer, pH 7.4.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
Daudi cells
其他说明
Kolberg, R., 1997. J. NIH Research9, 29.
Van Antwerp, D.J. et al. 1996. Science274, 787.
Beg, A.A., et al. 1995. Nature376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141.
Wulczyn, F.G., et al. 1992. Nature358, 597.
This antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
生物来源 | rabbit |
质量水平 | 100 |
antibody product type | primary antibodies |
克隆 | polyclonal |
形式 | liquid |
包含 | ≤0.1% sodium azide as preservative |
species reactivity | human |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
同位素/亚型 | IgG |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich