产品说明
特异性
NMDA receptor subunit 1 (all splice variants). No cross-reactivity observed with other NMDA receptor subunits. Recognizes a 103-116 kDa protein in rat brain hippocampal homogenate by immunoblot.
免疫原
Epitope: (all splice variants)
Fusion protein of amino acids 1-564 from rat NR1.
应用
Research Category
Neuroscience
Detect NMDAR1 using this Anti-NMDAR1 Antibody, (all splice variants), clone R1JHL validated for use in WB.
Western blot: 1:300. It is recommended that the antibody be diluted in TBST (20 mM Tris-HCl, 137 mM NaCl, 1% Tween-20, pH 7.6) containing 5% nonfat milk and 0.05% sodium azide. The diluted antibody can be used at least 5 times without a significant signal decrease. Store at 2-8°C between uses.
Immunoprecipitation: 3 μg per 200 μg of lysate will quantitatively precipitate all the NMDA R1 in 200 μg of rat brain lysate.
Optimal working dilutions must be determined by end user.
Research Sub Category
Neurotransmitters & Receptors
外形
Affinity purified immunoglobulin. Lyophilized. Reconstitute with 50 μL of PBS, pH 7.4. Contains no preservative.
Format: Purified
储存及稳定性
Maintain lyophilized material at -20°C for up to six months after date of receipt. After reconstitution, maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | R1JHL, monoclonal |
species reactivity | human, mouse, rat |
manufacturer/tradename | Chemicon® |
technique(s) | western blot: suitable |
同位素/亚型 | Not specified |
NCBI登记号 | NM_000832.5 NM_007327.2 NM_021569.2 |
UniProt登记号 | Q05586 |
运输 | dry ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich