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Anti-NONO antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
SAB4200502
规格纯度
clone NC5, purified from hybridoma cell culture
参考价格
5045.46 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-NONO antibody, Mouse monoclonal
Monoclonal Anti-NRB54
Monoclonal Anti-p54(nrb)
Monoclonal Anti-54 kDa nuclear RNA- and DNA-binding protein
Monoclonal Anti-52 kDa subunit
Monoclonal Anti-P54NRB
Monoclonal Anti-NONO antibody produced in mouse
Monoclonal Anti-55 kDa nuclear protein
Monoclonal Anti-DNA-binding p52/p100 complex
Monoclonal Anti-P54
Monoclonal Anti-non-POU domain-containing octamer (ATGCAAAT) binding protein
Monoclonal Anti-NMT55
产品介绍:

产品说明

一般描述

Monoclonal Anti-NONO (mouse IgG2a isotype) is derived from the hybridoma NC5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Non-POU-domain-containing, octamer binding protein (NONO), also known as p54nrb, is a member of the DBHS (Drosophila behavior, human splicing) protein family. The family also consists of paraspeckle protein 1 (PSPC1) and splicing factor proline- and glutamine-rich (SFPQ). NONO, PSPC1 and SFPQ are the core protein components of paraspeckles.

特异性

Monoclonal Anti-NONO recognizes mouse NONO.

免疫原

Synthetic peptide corresponding to a sequence at the C-terminal region of mouse NONO, conjugated to KLH.1 The corresponding sequence is identical in rat and differs by a single amino acids in human.

应用

Monoclonal Anti-NONO antibody produced in mouse has been used in immunohistochemistry and immunoblotting.

生化/生理作用

Non-POU-domain-containing, octamer binding protein (NONO) complexes with androgen receptor and activates androgen receptor-mediated transcription. NONO is necessary for cAMP-dependent activation of cAMP response element-binding protein (CREB) target genes. It acts as a bridge between the CREB/ transducers of regulated CREB (TORC) complex and RNA polymerase II. NONO associates with Period circadian protein homolog 1 (PERIOD-1) protein and is essential for the maintenance of circadian rhythm. NONO along with the nuclear factor, polypyrimidine tract-binding protein-associated splicing factor (PSF) binds to defective RNA and are capable of their retention.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

储存及稳定性

For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze at -20℃ in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified from hybridoma cell culture
antibody product typeprimary antibodies
克隆NC5, monoclonal
形式buffered aqueous solution
species reactivityrat, mouse
浓度~1.0 mg/mL
technique(s)indirect immunofluorescence: 1-2 μg/mL using whole extracts of mouse Hepa1-6 cells.
western blot: 0.5-1.0 μg/mL using whole extracts of mouse Hepa1-6 cells.
同位素/亚型IgG2a
UniProt登记号Q99K48
运输dry ice
储存温度−20℃
Gene Informationmouse ... Nono(53610)
rat ... Nono(317259)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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