产品说明
一般描述
Nuclease P1 (Nuclease 5′-Nucleotidehydrolase, 3′- Phosphohydrolase, EC 3.1.30.1), also known as deoxyribonuclease P1 or endonuclease P1, is a zinc dependent endonuclease. It is isolated from Penicillium citrinum.
免疫原
Nuclease P1 from Penicillium citrinum
应用
Anti-Nuclease P1 antibody produced in rabbit has been used in immunoblotting.
生化/生理作用
Nuclease P1 catalyzes the hydrolysis of 3′-5′ phosphodiester bonds in RNA and single-stranded DNA and 3′-phosphomonoester bonds in mono- and oligonucleotides terminated by a 3′-phosphate group without apparent bases specificity. The enzyme is used as a component of nuclease cocktail to generate single-stranded oligodeoxynucleotide (ODNs) samples for mass spectroscopy research for characterization of oligodeoxyribonucleotides. Nuclease P1 has also been widely used in the food industry to enhance or create flavor.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 抗体形式 | Ig fraction of antiserum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | ~30 kDa |
| 浓度 | ~1 mg/mL |
| technique(s) | immunoblotting: 1:10,000-1:20,000 using Nuclease P1 from Penicillium citrinum |
| UniProt登记号 | P24289 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
