产品说明
一般描述
Recognizes the ~53 kDa wild-type p53 and mutant p53 protein in SKBR-3 and MDA-231 cells and in colon carcinoma tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen. Recognizes the ~53 kDa wild-type and mutant forms of p53.
This Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-12) (Pantropic).
免疫原
recombinant, human p53 expressed in E. coli
Epitope: within amino acids 37-45 of p53
包装
100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
Frozen Sections (1-2 g/ml)
Immunoblotting (1-2 g/ml)
Immunofluorescence (0.5 g/ml)
Immunoprecipitation (2 g/mg lysate)
Paraffin Sections (1-2 g/ml)
警告
Toxicity: Standard Handling (A)
分析说明
Positive Control
SKBR-3 or MDA-231 cells or colon carcinoma tissue
其他说明
Exhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Lane, D.P. 1992. Nature358, 15.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | DO-7, monoclonal |
形式 | liquid |
不包含 | preservative |
species reactivity | bovine, monkey, human |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
同位素/亚型 | IgG2b |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich