Anti-Parvalbumin Antibody

产品说明

一般描述

Parvalbumin is a calcium binding albumin protein. It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is localised in fast-contracting muscles, where its levels are highest, and in the brain and some endocrine tissues. Parvalbumin is present in GABAergic interneurons in the nervous system, predominantly expressed by chandelier and basket cells in the cortex.

特异性

Parvalbumin. The antibody will react with parvalbumin from brain and muscle. By immunoblot it recognizes a protein of 12 kDa. The antibody is directed against an epitope at the first Ca+2-binding site and specifically stains the Ca+2 -bound form of parvalbumin.

免疫原

Parvalbumin purified from frog muscle

应用

Anti-Parvalbumin Antibody is an antibody against Parvalbumin for use in IC, IH(P) & WB.
Research Sub Category
Signaling Neuroscience
Research Category
Neuroscience
Immunohistochemistry:
A previous lot was used by indirect immunoperoxidase at 1:1,000-1:2,000 dilution. The antibody works on formalin fixed, paraffin embedded tissue sections of rat cerebellum from a previous lot. Fixation does not appear to affect MAB1572’s ability to detect parvalbumin.

Immunocytochemistry:
A previous lot of this antibody was used on cultured neurons.

Optimal working dilutions must be determined by the end user.

质量

Routinely evaluated by Western Blot on mouse brain lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected Parvalbumin on 10 μg of mouse brain lysate.

目标描述

12 kDa

外形

Unpurified
Ascites mouse monoclonal IgG1 liquid

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.

分析说明

Control
Brain tissue, Cultured neurons

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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