Anti-phospho-Keratin-18 Antibody (Ser52)

产品说明

一般描述

Keratin, type I cytoskeletal 18 (UniProt P05783; also known as Cell proliferation-inducing gene 46 protein, CK-18, Cytokeratin-18, K18, Keratin-18) is encoded by the KRT18 (also known as CYK18, PIG46) gene (Gene ID 3875) in human. Cytokeratin-18 (Keratin-18 or K18) belongs to a family of highly conserved epithelial-specific intermediate filament (IF) proteins that play an important role in the mechanical stability and integrity of epithelial cells and tissues. Each epithelium is characterized by its unique keratin expression pattern. All epithelial cells typically express at least one type-I and one type-II keratin, which form noncovalent heteropolymers. Keratin 18 is a type I intermediate filament protein that pairs with keratin 8 (K8/K18) in simple epithelia and plays an important role in protecting cells from stress, injury, and apoptosis. K18 is the only type-I keratin in adult hepatocytes and the major type-I keratin in many colonic tumor cell lines.The structure and function of K8/18 are regulated by posttranslational modifications, such as glycosylation, ubiquitination, and phosphorylation, including K8 phosphorylation on Ser8, Ser23, Ser73, Ser431, and K18 phosphorylation on Ser33 and Ser52. K18 phosphorylation has been proposed as a progression marker for human chronic hepatitis B (CHB). Progressively higher Ser52 phosphorylation is reported in non-cirrhotic and cirrhotic CHB livers, and progressive increase of Ser33 and Ser52 phosphorylation correlates with the elevation of both histological lesions and enzymatic activities of alanine aminotransferase in non-cirrhotic CHB livers.

特异性

Target phosphorylation site is not conserved in murine and rat species.
This rabbit polyclonal antibody detected upregulated cellular Keratin-18 Ser52 phosphorylation upon phosphatase inhibitors treatment. The Ser52 numbering is based on keratin-18 sequence without the initiation Met1, which was removed posttranslationally.

免疫原

KLH-conjugated linear peptide corresponding to human Keratin-18 target region sequence containing phosphorylated Ser52.

应用

This rabbit polyclonal Anti-phospho-Keratin-18 (Ser52) Antibody, Cat. No. ABT1383 is validated for use in Western Blotting for the detection of Keratin-18 Ser52 phosphorylation.

质量

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected upregulated Keratin-18 Ser52 phosphorylation in 10 µg lysate from phosphatase inhibitors Calyculin A- and Okadaic acid-treated HEK293 cells.

目标描述

~48 kDa observed. 47.93 kDa (Met1 removed) calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Affinity purified.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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