产品说明
一般描述
The SMAD3 (mothers against decapentaplegic homolog 3) gene is mapped to human chromosome 15q22.33. SMAD proteins contains three main domains : MH1 (MAD homology 1) - amino terminal domain (highly conserved), MH2 - carboxy terminal domain and a linker domain between the two domains. The MH1 domain of Smad3 serves as a DNA binding motif.(8)
免疫原
The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser204.
Immunogen Range: 170-219
应用
Anti-phospho-Smad3 (pSer204) antibody produced in rabbit has been used in western blot analysis.(7)
生化/生理作用
Smad3 (mothers against decapentaplegic homolog 3) protein identifies tandem repeats of the palindromic sequence GTCTAGAC, which is part of TGF-β (transforming growth factor β) signaling promoter region. Smad3 is associated with TGF-β (transforming growth factor beta) signaling pathway, a cellular process regulating the homeostasis, development, and repair of cartilage. It is known to induce extracellular matrix production. Smad proteins are responsible for the transduction of signals from cell surface to the nucleus. Phosphorylation of Smad3 controls tumor progression, inflammation, fibrosis, obesity and diabetes. Smad3 expression might be associated with the pathogenesis of osteoarthritis.
特点和优势
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外形
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 48 kDa |
| species reactivity | human, mouse, rat |
| 浓度 | ~1 mg/mL |
| technique(s) | ELISA: 1:20000 western blot: 1:500-1:1000 |
| NCBI登记号 | P84022 |
| UniProt登记号 | P84022 |
| application(s) | research pathology |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... SMAD3(4088) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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