产品说明
特异性
Antibody reacts with cytosolic form of PLA2. Antibody will also react with beta isoform. Epitope has been localized within the first 216 aa of the N-terminal Ca++ binding domain of cPLA2
免疫原
Recombinant cPLA2 expressed from the human cPLA2 gene.
应用
Research Category
Signaling
Research Sub Category
Lipid Signaling
Detect Phospholipase A2 using this Anti-Phospholipase A2 Antibody, clone CH-7 validated for use in ELISA & WB.
Immunoblot: 1:500-1:1000; antibody recognizes all isoforms, the cPLA.
Alpha (85kDa) typically migrates as 100-110kDa on reducing westerns; beta migrates as 114kDa; antibody will also recognize the sPLA at approximately 14-20kDa.
ELISA
Optimal working dilutions must be determined by the end user.
外形
In 20 mM sodium phosphate, 250 mM sodium chloride, 0.1% sodium azide, pH. 7.6
Format: Purified
储存及稳定性
Maintain at 2-8°C.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | CH-7, monoclonal |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable western blot: suitable |
同位素/亚型 | IgG2b |
NCBI登记号 | NM_000928.2 |
UniProt登记号 | P04054 |
运输 | wet ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich