您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2

Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2

品牌
Sigma-Aldrich
货号
MAB1059
规格纯度
clone 10F12.2, Chemicon®, from mouse
参考价格
5688.25 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2
pigment epithelium-derived factor, proliferation-inducing protein 35, serine (or cysteine) proteinase inhibitor, clade F (alpha-2

antiplasmin, pigment epithelium derived factor), member 1, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin
产品介绍:

产品说明

一般描述

Pigment epithelium-derived factor (PEDF) was first identified as a 50 kDa secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells (Tombran-Tink, 1991). More recently, PEDF was shown to have neurotrophic activity in the retina in vivo (Cayouette, 1999). PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells (Karakousis, 2001). The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. Recently, PEDF was shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo (Dawson, 1999; Stellmach, 2001).

特异性

Recognizes Pigment-Epithelium Derived Factor (PEDF).

免疫原

Recombinant human GST-PEDF fusion protein. The sequence corresponding to amino
acids 196-347 of human PEDF tagged with GST on the N-terminal was expressed in E. Coli. GST-PEDF is about 46 kDa on SDS-PAGE.

应用

Research Sub Category
Neurochemistry & Neurotrophins
Research Category
Neuroscience
Anti-Pigment Epithelium Derived Factor Antibody, clone 10F12.2 is an antibody against Pigment Epithelium Derived Factor for use in ELISA, IC, IH & WB.
Western Blot:
Recognizes a protein with a molecular weight of ~46 kDa when tested against immunogen. Western blot positive control is available (Catalog number AG672)

ELISA:
A previous lot of this antibody was used on ELISA.

Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemistry.

Immunohistochemistry:
A previous lot of this antibody was used on frozen sections.

Optimal working dilutions must be determined by end user.

质量

Routinely evaluated by Western Blot on human placenta lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected pigment epithelium on 10 μg of human placenta lysate.

目标描述

~46 kDa

外形

Purified mouse monoclonal IgG2a in buffer containing 0.02 M PBS, pH 7.6, 0.25 M NaCl with 0.1% sodium azide.
Format: Purified
Protein A purified

储存及稳定性

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

分析说明

Control
Eye tissue.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆10F12.2, monoclonal
species reactivityhuman
species reactivity (predicted by homology)rat
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型IgG2a
NCBI登记号NM_002615.4
UniProt登记号P36955
运输wet ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品