产品说明
一般描述
Monoclonal Anti-PIWIL1 (mouse IgG1 isotype) is derived from the hybridoma PIWIL1-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to keyhole limpet hemocyanin (KLH). The PIWI-like protein 1 (PIWIL1) belongs to the Argonaute protein family. This gene is located on human chromosome 12q24.33. PIWIL1 protein has 861 amino acid residues and possess a molecular weight of 98.6?kDa. It is expressed in prostate, ovary, brain, liver, heart, kidney and skeletal muscle.
免疫原
synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence is identical in mouse PIWIL1.
应用
Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in various immunochemical techniques including immunoblotting (~95 kDa) and immunohistochemistry.
生化/生理作用
Piwi proteins and piRNAs have been implicated in epigenetic control of gene expression, transposon silencing and translation regulation. Piwi proteins participates in germline development and gametogenesis of many metazoan species. PIWI-like protein 1 (PIWIL1) is also involved in stem cell proliferation, embryogenesis, growth and development, along with differentiation and maturation in numerous organisms. It can act as a molecular marker for forecasting the prognosis of colorectal cancer (CRC) patients.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
法律信息
ExtrAvidin is a registered trademark of sigma-aldrich Co. LLC
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | PIWIL1-6, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~95 kDa |
| species reactivity | mouse, human |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidin®-Peroxidase staining system western blot: 2.5-5.0 μg/mL using whole extracts of mouse testis or HEK-293T cells over-expressing PIWIL1 |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q96J94 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... PIWIL1(9271) mouse ... Piwil1(57749) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
