Anti-PL Scramblase 1 Antibody, clone 9A7

产品说明

一般描述

Phospholipid scramblase 1 (UniProt Q9JJ00; also known as Ca(2+)-dependent phospholipid scramblase 1, Nor1, PL scramblase 1, Tra1, Transplantability-associated protein 1) is encoded by the Plscr1 (also known as Tra1b, Tras1) gene (Gene ID 22038) in murine species. Plasma membrane phospholipids are distributed asymmetrically between the inner and outer leaflets. Such asymmetrical distribution collapses in response to blood coagulation and apoptosis, resulting in phospholipid “scrambling” between the two leaflets of the plasma membrane. Flippases, floppases, and scramblases are three types enzymes known to mediate transbilayer lipid motion. Flippases and floppases function via ATP-dependent mechanism, while scramblases mediate transbilayer movement in a non-selective and energy-independent manner. Originally identified in 1996 as a 37 kDa erythrocyte type II transmembrane protein that mediates calcium-dependent membrane phospholipids redistribution, PL Scramblase 1 is the protein product encoded by the founding member of the PLSCR family of genes (PLSCR1-5). All PLSCR family members, with the exception of PLSCR2, possess a proline-rich N-terminal region containing PxxP and PPxY domains, a cysteine-rich region, a conserved calcium-binding domain (EF-hand-like), and a putative transmembrane region enriched in hydrophobic amino acids. In addition, PLSCR1 contains a nuclear localization signal (NLS) and a DNA-binding domain that are essential for its nuclear localization and associated nuclear function. In addition to PLSCRs, TMEM16 and XKR family members have also been reported to mediate scramblase activity.

特异性

Clone 9A7 reacts with murine PL Scramblase 1, and does not cross-reacts with murine PL Scramblase 2/3/4 or human PL Scramblase 1.

免疫原

Recombinant protein corresponding to the cytoplasmic domain of mouse PL Scramblase 1.
Epitope: Cytoplasmic domain

应用

Research Category
Cell Structure
Anti-PL Scramblase 1 Antibody, clone 9A7 is an antibody against PL Scramblase 1 Antibody for use in Western Blotting.
Western Blotting Analysis: A representative lot detected PL Scramblase 1 in mouse lung homogenates. Clone 9A7 also detected LPS treatment-induced PL Scramblase 1 upregulation in liver using total tissue homogenates, as well as cytosolic and nuclear extracts from LPS-treated and untreated mice (Lu, B., et al. (2007). Biochim. Biophys. Acta. 1771(9):1177-1185).
Research Sub Category
Adhesion (CAMs)

质量

Evaluated by Western Blotting in mouse thymus tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected PL Scramblase 1 in 10 µg of mouse thymus tissue lysate.

目标描述

~38 kDa observed. Two additional bands shown correspond to heavy and light chains of endogenous IgG from the mouse thymus tissue.

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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