Anti-Rabies Virus Antibody, clone 1112-1

产品说明

一般描述

Glycoprotein of Evelyn Rokitniki Abelseth (ERA) SAD derived Rabies virus strain (UniProt: P03524) is encoded by the G gene. It belongs to the lyssavirus glycoprotein of the Rhabdoviridae family. This glycoprotein is a single pass type I virion membrane protein that helps in attaching the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. It has been shown that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (N-CAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and facilitate rabies virus entry into cells. Arg352 of Glycoprotein is highly involved in rabies virus pathogenicity and mutation at this site dramatically attenuates the virus.

Glycoprotein of Evelyn Rokitniki Abelseth (ERA) SAD derived Rabies virus strain (UniProt: P03524) is encoded by the G gene. It belongs to the lyssavirus glycoprotein of the Rhabdoviridae family. This glycoprotein is a single pass type I virion membrane protein that helps in attaching the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. It has been shown that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (N-CAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and facilitate rabies virus entry into cells. Arg352 of Glycoprotein is highly involved in rabies virus pathogenicity and mutation at this site dramatically attenuates the virus.

特异性

Clone 1112-1 detects glycoprotein of ERA strain of rabies virus in L929 cells that express rabies protein.

Clone 1112-1 detects glycoprotein of ERA strain of rabies virus in L929 cells that express rabies protein.

免疫原

Glycoprotein of ERA (Evelyn Rokitniki Abelseth) strain Rabies virus.

Glycoprotein of ERA (Evelyn Rokitniki Abelseth) strain Rabies virus.

应用

Neutralizing Analysis: A representative lot neutralized Rabies Virus (Bunschoten, H., et. al. (1989). J Gen Virol. 70 ( Pt 2):291-8; Dietzchold, B., et. al. (1992). Proc Natl Acad Sci USA. 89(15):7252-6; Muller, T., et. al. (2009). PLoS Neql Trop Dis. 3(11):e542; Schumacher, C.L., et. al. (1989). J Clin Invest. 84(3):971-5).

Anti-Rabies Virus, clone 1112-1, Cat. No. MABF949, is a mouse monoclonal antibody that detects glycoprotein of strain ERA rabbies virus. It has been tested for use in Flow Cytometry and for Neutralizing.
Neutralizing Analysis: A representative lot neutralized Rabies Virus (Bunschoten, H., et. al. (1989). J Gen Virol. 70 ( Pt 2):291-8; Dietzchold, B., et. al. (1992). Proc Natl Acad Sci USA. 89(15):7252-6; Muller, T., et. al. (2009). PLoS Neql Trop Dis. 3(11):e542; Schumacher, C.L., et. al. (1989). J Clin Invest. 84(3):971-5).
Research Category
Inflammation & Immunology

质量

Evaluated by Flow Cytometry in L929 expressing rabies protein.

Flow Cytometry Analysis: 1 µg of this antibody detected Rabies Virus in one million L929 cells expressing rabies protein.

目标描述

58.66 kDa calculated.

外形

Protein G purified
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Format: Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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