Anti-RAGE Antibody, clone DD/A11 or A11

产品说明

一般描述

RAGE (Receptor for Advanced Glycosylation End Products) is a 35 kDa cell surface receptor that binds molecules modified by advanced glycation end products (AGEs), an irreversible non-enzymatic reaction occuring from the interaction of proteins and lipids with glucose. The increased expression of RAGE is associated with many disorders, such as diabetic vasculopathy, neuropathy, retinopathy and neuropathy, Alzheimer’s disease and immune/inflammatory reactions of the vessel walls. AGE/RAGE accumulates at sites of vascular disease in diabetes, but blockade of RAGE suppresses development of atherosclerosis and vascular disorders. RAGE binds to amyloid beta, which is overproduced in neurons and vessels in Alzheimer′s disease, and this interaction results in oxidative stress leading to neuronal degeneration.

特异性

RAGE (Receptor for Advanced Glycosylated End Products). The antibody recognizes both natural and recombinant RAGE.

免疫原

Recombinant truncated extracellular part of RAGE produced in E. coli.

应用

Immunohistochemistry:
A previous lot of this antibody was shown to be reactive on paraffin embedded tissue sections.

Immunocytochemistry:
A previous lot was shown to work on cells fixed with ice cold acetone using a Cy3 conjugated secondary antibody.

ELISA:
A previous lot was shown to work on direct ELISA.

Immunoblotting:
Recognizes a band of ~48kDa in bovine lung extract.

Optimal working dilutions must be determined by end user.
Anti-RAGE Antibody, clone DD/A11 or A11 is an antibody against RAGE for use in ELISA, IC, IH & WB.

质量

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot:
1:500 dilution of this lot detected RAGE on 10 μg of Mouse Brain lysates.

目标描述

48 kDa

外形

Format: Purified
Purified mouse monoclonal IgG2a in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, and 0.1% sodium azide.
Protein A purified

分析说明

Control
Lung tissue, mouse brain lysate.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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