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位置:首页 > 品牌 > Sigma-Aldrich > Anti-Reelin Antibody, a.a. 164-189 mreelin, clone 142

Anti-Reelin Antibody, a.a. 164-189 mreelin, clone 142

品牌
Sigma-Aldrich
货号
MAB5366
规格纯度
clone 142, Chemicon®, from mouse
参考价格
6526.19 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Anti-Reelin Antibody, a.a. 164-189 mreelin, clone 142
Reelin
产品介绍:

产品说明

一般描述

The highly layered structure of the cerebral cortex is established through the pattern of neuronal cell migrations. The first step is the creation of the primordial layer, the preplate, consisting of radial glial cells and the earliest generated neurons. Among these neurons are the Cajal-Retzius neurons. In the next step, the preplate splits into a superficial (marginal) zone, where the Cajal-Retzius neurons reside, and a deep subplate wherein the neurons form. Neurons migrating from the subplate form the cortical plate. This migration takes place on the radial glial fibers.



The reeler mutant in mouse displays an abnormal pattern of cell migration throughout the cerebral and cerebellar cortices. The preplate forms normally, and the neurons differentiate at the correct times in the ventricular zone. However, instead of forming the normal "inside-out" arrangement of neurons in the cortical plate, the older neurons are found furthest from the ventricular zone, while the younger neurons do not migrate far at all. The reeler cerebral cortex is inverted from that of the wild type mouse.



The defect of the reeler mice appears to be in the production of an extracellular matrix protein by the Cajal-Retzius cells (D′Arcangelo et al., 1995, Nature 374:719-723.; Ogawa et al., 1995 Neuron 14:899-912.) This 388kDa protein is made by wild-type mice but not by the reeler mutants. It is thought that this Reelin protein is crucial for positioning the migrating neuron within the cortical plate (Figure 1). In the absence of Reelin, the migrating neuron would be "lost," and the cortical plate would be abnormal. We do not yet know the mechanisms by which Reelin informs the cells as to their position, how the cell responds to Reelin, and why the absence of reelin should give an "inverted" plate. However, the identification of the protein encoded by the reeler gene should allow us to begin these studies.

特异性

Reelin. The epitope is between amino acids 164 and 189.

免疫原

Epitope: a.a. 164-189
Recombinant reelin amino acids 40-189.

应用

Western blot:
1:250-1:500, In Western blots, reelin appears as three bands ~400 - 450, 300, and 180 - 200 kDa′s.

Immunohistochemistry:
1:250-1:500, 4% PFA fixed tissues. A previous lot of this antibody was used in IH.

Optimal working dilutions must be determined by the end user.
This Anti-Reelin Antibody, a.a. 164-189 mreelin, clone 142 is validated for use in IH, WB for the detection of Reelin.
Research Category
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance

质量

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected Reelin on 10 μg of mouse brain lysates.

目标描述

In Western blots, reelin can appear as three bands ~400 - 450, 300, and 180 - 200 kDa′s.

外形

Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
Protein A purified

储存及稳定性

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.

分析说明

Control
Mouse liver or kidney

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆142, monoclonal
species reactivityvertebrates, mouse, human
species reactivity (predicted by homology)rat
manufacturer/tradenameChemicon®
technique(s)immunohistochemistry: suitable
western blot: suitable
同位素/亚型IgG1
NCBI登记号NM_005045.2
UniProt登记号P78509
运输wet ice
Gene Informationhuman ... RELN(5649)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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