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Anti-Replication Protein A Antibody, clone RPA34-20

品牌
Sigma-Aldrich
货号
MABE285
规格纯度
clone RPA34-20, from mouse
参考价格
6022.61 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Replication Protein A Antibody, clone RPA34-20
Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34
产品介绍:

产品说明

一般描述

RPA (RP-A p32) is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits: RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa). RPA plays multiple roles in DNA replication. At the onset of DNA replication, RPA is loaded onto chromatin, and is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination. The 32 kDa subunit is phosphorylated by the cdc2 family of kinases when cells enter S-phase; and by ATM, ATR, and DNA-PK proteins in response to DNA damage.

免疫原

Replication Protein A purified from U293 cells

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-Replication Protein A Antibody, clone RPA34-20 (mouse monoclonal antibody) validated in WB, ICC, IHC to detect Replication Protein A also known as Replication protein A 32 kDa subunit, RP-A p32.
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Replication Protein A in HeLa and A431 cells.

Immunohistochemistry Analysis: A 1:5 dilution from a representative lot detected Replication Protein A in human colorectal adenocarcinoma tissues.

质量

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Replication Protein A in 10 µg of HeLa cell lysate.

目标描述

~30 kDa observed. Uniprot describes three isoforms at ~29 kDa, ~30 kDa, and ~39 kDa

联系

Replaces: NA19L

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa cell lysate.B10

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆RPA34-20, monoclonal
species reactivityhuman
technique(s)immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_002937
UniProt登记号P15927
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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