Anti-Rhodopsin Antibody, CT, last 9 amino acids, clone Rho 1D4

产品说明

特异性

Recognizes Rhodopsin. Reacts with both native and recombinant protein. No known reactivity to other proteins.

免疫原

Epitope: C-terminus, last 9 amino acids
Bovine rhodopsin

应用

Anti-Rhodopsin Antibody, C-terminus, last 9 amino acids, clone Rho 1D4 detects level of Rhodopsin & has been published & validated for use in IC, IH, IP & WB.
Research Sub Category
Sensory & PNS
Research Category
Neuroscience
Western blot: 1:100-1:500 using ECL. Suggested dilution buffer is PBS and blocking buffer is PBS containing 5% milk. Recognizes 36 kDa monomers, dimmers and trimers depending on sample preparation. Boiling induces the aggregation of rhodopsin.

Western blot of isolated bovine rod outer segment labeled for rhodopsin (~85% of rod outer segment protein) with the rho 1D4 antibody. Amount of rod outer segments applied to the SDS gel: (a) 2.5 mg; (b) 0.63 mg; (c) 0.16 mg; (d) 0.04 mg. At higher protein quantities, dimers, trimers and tetramers of rhodopsin can be observed along with the monomer.

Immunohisto/cytochemistry: 1:100-1:500 on paraformaldehyde and glutaraldehyde fixed frozen tissue sections. Preferred fixation is paraformaldehyde for 4 hours at 2-8°C. Suggested permeabilization method is 0.2% Triton X-100.

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

目标描述

48 kDa

外形

Format: Purified
Protein A purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

储存及稳定性

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Eye

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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