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Anti-Ribophorin I/RPN-I Antibody

品牌
Sigma-Aldrich
货号
ABS1587
规格纯度
serum, from rabbit
参考价格
5520.38 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Ribophorin I/RPN-I Antibody
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1
产品介绍:

产品说明

一般描述

Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 1 (EC 2.4.99.18; UniProt P04843; also known as Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1) is encoded by the RPN1 gene (Gene ID 6184) in human. Ribophorin I is an ER transmembrane protein and a subunit of the mammalian Oligosaccharyltransferase (OST) complex. Ribophorin I binds newly synthesized target proteins and delivers them to the catalytic core of the OST complex. Association of ribophorin I with newly synthesized membrane proteins was observed after the exit of the nascent chain from the Sec61 translocon, and it was unaffected by the N-glycosylation status. Ribophorin I is reported to preferentially interact with misfolded over native ribonuclease A. In addition, Ribophorin I is shown to complex with another ER protein malectin and enhance malectin′s ability to prevent the secretion of the folding-defective proteins.

免疫原

KLH-conjugated linear peptide corresponding to the C-terminal sequence of human Ribophorin I/RPN-I.
Epitope: C-terminus.

应用

This Anti-Ribophorin I/RPN-I Antibody is validated for use in Western Blotting for the detection of Ribophorin I.
Research Category
Signaling
Research Sub Category
Organelle & Cell Markers
Western Blotting Analysis: An 1:5,000 dilution from a representative lot detected ribophorin I in in 25 µg of HeLa cell lysate (Courtesy of Dr. Christopher V. Nicchitta, Duke University Medical Center, Durham, NC).
Western Blotting Analysis: A representative lot detected ribophorin I in the alkali-extracted and Triton X-114-selected integral membrane fraction derived from UV-cross-linked canine pancreas rough microsomes (RM) preparation (Jagannathan, S., et al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924).
Western Blotting Analysis: A representative lot detected ribophorin I in both the Brij35-sensitive (BrS) and Brij35-resistant (BrR) fraction of HeLa ER membrfane proteins (Jagannathan, S., et al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924).
Western Blotting Analysis: A representative lot detected ribophorin I associated with the rRNA-free poly(A) messenger ribonucleoproteins (mRNPs) purified by oligo(dT) from UV-cross-linked canine pancreas rough microsomes (RM) preparation (Jagannathan, S., et al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924).
Western Blotting Analysis: A representative lot detected ribophorin I in both the smooth and rough microsomes sub-fractionated from a rat liver microsome preparation (Menon, A.K., et al. (2000). Curr. Biol. 10(5):241-252).
Western Blotting Analysis: A representative lot detected ribophorin I in canine rough microsome (RM) fractions obtained by sucrose gradient centrifugation of digitonin- or DHPC-solubilized canine RM preparation with or without ribosomes stripping by EDTA treatment (Potter, M.D., and Nicchitta, C.V. (2000). J. Biol. Chem. 275(3):2037-2045).

质量

Evaluated by Western Blotting in canine pancreas tissue rough microsome lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Ribophorin I/RPN-I in 2.5 µL of canine pancreas tissue rough microsome lysate.

目标描述

~68 kDa observed.

外形

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式serum
antibody product typeprimary antibodies
克隆polyclonal
species reactivitycanine, rat, human
species reactivity (predicted by homology)porcine (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology), monkey (based on 100% sequence homology)
technique(s)western blot: suitable
NCBI登记号NP_002941
UniProt登记号P04843
运输dry ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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