产品说明
一般描述
Rab3-interacting molecules (RIMs) are synaptic proteins necessary for neuronal transmission and plasticity. RIM 1 and RIM 2 proteins are expressed in overlapping but distinct patterns throughout the brain. While the ablation of either gene was not lethal in mice, the deletion of both resulted in postnatal mortality. This lethality is due to a defect in neurotransmitter release; synapses without RIM proteins can still release neurotransmitters but are unable to do so in response to normal Ca2+ triggers. Like RIM 1, RIM 2 is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner.
特异性
This antibody may recognize many isoforms and is predicted to have no cross-reactivity to other RIM proteins.
免疫原
Epitope: center region
KLH-conjugated synthetic peptide corresponding to the center region of human RIM 2.
应用
Anti-RIM 2 Antibody is an antibody against RIM 2 for use in western blotting, IHC & immunofluorescence.
Immunohistochemistry Analysis: 5 μg/mL from a representative lot detected RIM 2 in human brain tissue.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected RIM 2 in human brain cells.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
质量
Evaluated by Western Blotting in rat brain tissue lysate with and without blocking peptide.
Western Blotting Analysis: 1 μg/mL of this antibody detected RIM 2 in rat brain tissue lysate with and without blocking peptide.
目标描述
~86 kDa observed. Uniprot describes 3 isoforms produced by alternative splicing ranging between ~31 kDa and ~160 kDa
外形
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Antigen Affinity Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | rat, human |
technique(s) | immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable |
UniProt登记号 | Q9UQ26 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich