Anti-Robo2 Antibody, clone 4C6.1

产品说明

一般描述

Roundabout homolog 2 (Robo2) is a receptor for SLIT2, and likely SLIT1, which are thought to help guide cellular migration, including axonal navigation of the neural tube and the projection of axons during neuronal development. Vesicoureteral reflux type 2 (VUR2) has been attributed to defects in Robo2.

特异性

This antibody recognizes the cytoplasmic domain of Robo2

免疫原

GST-tagged recombinant protein corresponding to the cytoplasmic domain of human Robo2.
Epitope: Cytoplasmic domain

应用

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Robo2 in rat brain (hypothalamus) and human brain tissue.
Research Category
Neuroscience
Detect Roundabout homolog 2 using this mouse monoclonal antibody, Anti-Robo2 Antibody, clone 4C6.1 validated for use in western blotting & IHC (Paraffin).
Research Sub Category
Developmental Signaling

质量

Evaluated by Western Blotting in mouse neuronal stem cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Robo2 in 200 µg of mouse neuronal stem cell lysate.

目标描述

~ 170 kDa observed. The calculated molecular weight is 150 kDa, however Robo2 has been shown as a ~170 kDa band in western blots due to high glycosylation. (Fricke, C., et al. (2001). Science. 292:(5516):507-510).

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse neuronal stem cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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