Anti-Ser/Thr-protein kinase Nek2 Antibody, clone 5D2.1

产品说明

一般描述

Serine/threonine-protein kinase Nek2 (EC 2.7.11.1; UniProt P51955; also known as HSPK 21, Never in mitosis A-related kinase 2, NimA-like protein kinase 1, NimA-related protein kinase 2, Protein phosphatase 1 regulatory subunit 111) is encoded by the NEK2 (also known as NEK2A, NLK1, PPP1R111, RP67) gene (Gene ID 4751) in human. Nek2 is a member of the never in mitosis A- (NIMA-) related family of serine/threonine kinases. Nek2 is involved in cell cycle regulation, gene expression, and the maintenance of centrosomal structure and function. Cellular Nek2 levels are tightly regulated throughout the cell cycle with noncentrosomal Nek2 expression peaks from S to G2 phase, while cytoplasmic Nek2 is highest in G1, and nuclear Nek2 is highest during S and G2 phase when it is needed for centrosomal function regulation. During interphase, centrosomes are held together by a proteinaceous linker that connects the the mother and daughter centriole. This linker is disassembled at the onset of mitosis in a process known as centrosome disjunction. The mammalian sterile 20-like kinase 2 (Mst2) and the scaffold protein Salvador (hSav1) are shown to directly interact with Nek2A and regulate its ability to localize to centrosomes, where Nek2A mediating linker dissociation by phosphorylating the centrosomal linker proteins C-Nap1 and rootletin. In addition, Nek2 is also reported to regulate gene expression by phosphorylating the splicing factor SRSF1. Nek2 expression is found upregulated in a wide variety of neoplasms including diffuse large B cell lymphoma and multiple myeloma.

特异性

Expected to react with all four spliced isoforms of human Ser/Thr-protein kinase Nek2.

免疫原

Epitope: N-terminal half
GST-tagged recombinant protein corresponding to the N-terminal of human Ser/Thr-protein kinase Nek2.

应用

Research Sub Category
Nuclear Receptors
This Anti-Ser/Thr-protein kinase Nek2 Antibody, clone 5D2.1 is validated for use in Western Blotting, Immunocytochemistry for the detection of Ser/Thr-protein kinase Nek2.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Ser/Thr-protein kinase Nek2 in 10 µg of MOLT-4 and HL-60 cell lysates.
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Ser/Thr-protein kinase Nek2 in HeLa, NIH/3T3, and HUVEC cells.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Ser/Thr-protein kinase Nek2 in 10 µg of THP-1 cell lysate.

目标描述

~52/44 kDa observed (51.8 kDa/Nek2A (isoform 1), 44.9 kDa/Nek2B (isoform 2), 38.8 kDa/isoform 3, 50.9 kDa/Nek2C (isoform 4 or Nek2A-T), calculated). Uncharacterized band(s) may appear in some lysates.

外形

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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