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Anti-SMARCAL1 Antibody

品牌
Sigma-Aldrich
货号
ABE1836
规格纯度
serum, from rabbit
参考价格
5016.79 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-SMARCAL1 Antibody
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, HepA-related protein, hHARP, Sucrose nonfermenting protein 2-like 1, SMARCAL1
产品介绍:

产品说明

一般描述

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 (UniProt Q9NZC9; also known as ATP-driven annealing helicase, HepA-related protein, hHARP, SMARCA-like protein 1, Sucrose nonfermenting protein 2-like 1) is encoded by the SMARCAL1 (also known as HARP, HHARP) gene (Gene ID 50485) in human. The annealing helicase SMARCAL1 is a replication stress response protein that maintains genome integrity at stalled replication forks during DNA replication. SMARCAL1 binds Replication protein A (RPA) via its RPA-binding motif, which is both necessary and sufficient to target SMARCAL1 to stalled replication forks. Although not necessary for SMARCAL1 enzymatic activity, RPA binding is critical for its cellular function. Ataxia-telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) phosphorylate SMARCAL1 in response to replication stress. SMARCAL1-deficient cells are hypersensitive to replication stress agents and SMARCAL1 gene mutations cause Schimke immunoosseous dysplasia (SIOD).

特异性

Reacts with both human and murine SMARCAL1. Expected to react with all four spliced isoforms of murine SMARCAL1.

免疫原

Recombinant protein corresponding to the N-terminus of human SMARCAL1.

应用

Immunoprecipitation Analysis: 3.0 µL from a representative lot immunoprecipitated SMARCAL1 in HEK293T cell lysate (Data courtesy of Dr. David Cortez, Vanderbilt University).
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected SMARCAL1 in siRNA transfected HEK293T cell lysate (Data courtesy of Dr. David Cortez, Vanderbilt University).
Western Blotting Analysis: A representative lot detected SMARCAL1 in U2OS cell lysate (Bansbach, C.E., et al. (2009). Genes Dev. 23(20):2405-2414).
Anti-SMARCAL1 Antibody, Cat. No. ABE1836, is a highly specific rabbit Polyclonal antibody, that targets SMARCAL1 and has been tested in Western Blotting, Immunoprecipitation.

质量

Evaluated by Western Blotting in mouse myoblast C2C12 nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected SMARCAL1 in 10 µg of mouse myoblast C2C12 nuclear extract.

目标描述

~110 kDa observed. Human SMARCAL1 (105.9 kDa calculated). Mouse SMARCAL1 (100.8 kDa/isoform 1, 87.1 kDa/isoform 2, 63.2 kDa/isoform 3, 44.2 kDa/isoform 4 calculated). Uncharacterized band(s) may appear in some lysates.

外形

Unpurified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式serum
antibody product typeprimary antibodies
克隆polyclonal
species reactivityhuman, mouse
technique(s)immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号NP_001120679
UniProt登记号Q9NZC9
运输wet ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1

Sigma-Aldrich

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