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Anti-Talin antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
SAB4200694
规格纯度
clone 8D4, purified from hybridoma cell culture
参考价格
6732.28 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Talin antibody, Mouse monoclonal
Tln
产品介绍:

产品说明

一般描述

Anti-Talin antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma 8D4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified cytoskeletal Talin, from chicken gizzard. Talin is a high molecular weight cytoplasmic adapter protein. It is localized at a subset of adherens junctions. Talin contains an N-terminal FERM domain (the head) linked to a flexible rod comprised of 13 amphipathic helical bundles and a C-terminal helix (DD) that forms an anti-parallel dimer. It may be found either as monomer or dimer.

免疫原

purified cytoskeletal Talin, from chicken gizzard.

应用

Anti-Talin antibody, Mouse monoclonal has been used in:

  • immunoblotting
  • immunofluorescence
  • fluorescence-activated cell sorting (FACS)
  • enzyme-linked immunosorbent assay (ELISA)

生化/生理作用

Talin is essential for integrin-mediated adhesion to the extracellular matrix (ECM). Talin links the actin cytoskeleton to integrins at the plasma membrane and presents in a variety of tissues and cell types. In cultured cells, Talin is absent in cell-cell junctions and is found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin contains multiple binding sites for the cytoskeletal protein, vinculin. It is considered as a mechanosensor, undergoing a progressive mechanoresponse to the varying forces exerted throughout adhesion and migration, such as conformational changes, disrupted or exposed ligand binding sites and exposing hidden protease cleavage sites.

外形

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.41

产品性质

质量水平200
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆8D4, monoclonal
形式buffered aqueous solution
species reactivitychicken, human, sheep, hamster, Xenopus, rat, bovine
浓度~1 mg/mL
technique(s)ELISA: suitable
flow cytometry: suitable
immunoblotting: 1-2 μg/mL using whole extract of hamster CHO cells.
immunofluorescence: 2.5-5 μg/mL using chicken UMNSAH/DF1 cells.
同位素/亚型IgG1
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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