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Anti-TIM4/TIMD-4 Antibody, clone Kat5-18

品牌
Sigma-Aldrich
货号
MABC958
规格纯度
clone Kat5-18, from hamster(Armenian)
参考价格
6694.05 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-TIM4/TIMD-4 Antibody, clone Kat5-18
T-cell immunoglobulin and mucin domain-containing protein 4, TIMD-4, Spleen, mucin-containing, knockout of lymphotoxin protein, SMUCKLER, T-cell immunoglobulin mucin receptor 4, TIM-4, T-cell membrane protein 4, TIM4/TIMD-4
产品介绍:

产品说明

一般描述

T-cell immunoglobulin and mucin domain-containing protein 4 (UniProt Q6U7R4; also known as Spleen mucin-containing knockout of lymphotoxin protein, T-cell immunoglobulin and mucin domain containing 4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, TIMD-4, Smuckler) is encoded by the Timd4 (also known as B430010N18Rik, Tim4) gene (Gene ID 276891) in murine species. Phagocytes, including macrophages, target apoptotic cells for engulfment by recognizing their surface exposed phosphatidylserine (PtdSer or PS). Macrophages employ specific receptors and opsonins for PS recognition, such as Milk-fat globule epidermal growth factor 8 (MFG-E8), protein S, growth arrest-specific 6 (Gas6), and TAM family TKR (Tyro 3, Axl, and MerTK) that function as protein S and Gs6 receptors. Tim (T-cell immunoglobulin and mucin domain-containing) family proteins, stabilins, and BAI1 also directly bind PtdSer and enhance the engulfment of apoptotic cells by phagocytes. Tim4 is shown to mediate murine resident peritoneal macrophages (rpMacs) engagment of apoptotic cells, while MerTK-mediates the engulfment of tethered target cells. Tim4- or MerTK-null mutations prevent rpMac-mediated apoptotic cell engulfment. Tim4-null, but not MerTK-null, macrophages lose their ability to tether apoptotic cells. Murine Tim4 is initially produced with a signal peptide sequence (a.a. 1-22), the removal of which yields the mature Tim4 with a large extracellular region (a.a.23-279), a transmembrane domain (a.a. 280-300), and a short cytoplasmic tail (a.a. 301-343).

免疫原

Mouse peritoneal cells.
Epitope: Extracellular domain

应用

Research Sub Category
Apoptosis - Additional
Flow Cytometry Analysis: A representative lot detected Tim4 immunoreactivity among 61% of the resident peritoneal Tim4+ MerTK+ macrophages from C57BL/6J mice (Nishi, C., et al. (2014). Mol. Cell. Biol. 34(8):1512-1520).
Flow Cytometry Analysis: Representative lots were conjugated with biotin and detected Tim4 immunoreactivity among mouse Mac1+ peritoneal cells (Miyanishi, M., et al. (2012). Int. Immunol. 24(9):551-559; Miyanishi, M., et al. (2007). Nature. 450(7168):435-439).
Neutralizing Analysis: A representative lot blocked Tim4-mediated engulfment of apoptotic CAD-/- thymocytes by murine peritoneal macrophages in a dose-dependent manner in culture (Miyanishi, M., et al. (2007). Nature. 450(7168):435-439).
Neutralizing Analysis: A representative lot, when administered via i.v. injection, significantly suppressed the phagocytosis activity of F40/80+ macrophages in the thymus of CAD-/- mice following intraperitoneal dexamethasone injection to induce apoptosis in the thymus (Miyanishi, M., et al. (2007). Nature. 450(7168):435-439).
Research Category
Apoptosis & Cancer
This Anti-TIM4/TIMD-4 Antibody, clone Kat5-18 is validated for use in Flow Cytometry and Neutralizing for the detection of TIM4/TIMD-4.

质量

Evaluated by Flow Cytometry in Ba/F3-Tim4 cells overexpressing mouse Tim4.

Flow Cytometry Analysis: 0.1 µg of this antibody detected TIM4/TIMD-4 in Ba/F3-Tim4 cells overexpressing mouse Tim4.

目标描述

~37 kDa calculated

外形

Purified armenian hamster monoclonal IgG antibody in PBS without preservatives.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

产品性质

生物来源hamster (Armenian)
质量水平100
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆Kat5-18, monoclonal
species reactivitymouse
technique(s)flow cytometry: suitable
neutralization: suitable
同位素/亚型IgG
NCBI登记号NP_848874
UniProt登记号Q6U7R4
运输dry ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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