产品说明
一般描述
Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). Embryonal carcinoma, as do all human pluripotent stem cells, expresses TRA-1-60 and TRA-1-81 antigens, and although their molecular identities are unknown, they are commonly used as markers of undifferentiated pluripotent human stem cells.
特异性
This antibody reacts with TRA-1-81 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-81 and TRA-1-60 monoclonal antibodies (MAB4360) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.
免疫原
Human embryonal carcinoma cell line 2102Ep
应用
Immunofluorescence: A previous lot of this antibody was used in IF.
Western Blot: A previous lot of this antibody was used in WB.
Immunoprecipitation: A previous lot of this antibody was used in IP.
Immunohistochemistry: A previous lot of this antibody was used in IH.
Flow Cytometry: A starting range of 10-20 µg/mL is suggested.
Optimal working dilutions must be determined by the end user.
Research Sub Category
Pluripotent & Early Differentiation
Anti-TRA-1-81 Antibody, clone TRA-1-81 detects level of TRA-1-81 & has been published & validated for use in WB, FC, IF, IP & IC.
Research Category
Stem Cell Research
目标描述
420/250 kDa
外形
Liquid in 0.05 M Potassium Phosphate, 0.3 M NaCl, pH 8.0 with 0.05% Sodium Azide.
Format: Purified
Ammonium Sulfate Precipitation
储存及稳定性
Stable for 1 year at from date of receipt.
分析说明
Control
Human embryonic stem cells
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | saturated ammonium sulfate (SAS) precipitated |
antibody product type | primary antibodies |
克隆 | TRA-1-81, monoclonal |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | flow cytometry: suitable immunocytochemistry: suitable immunofluorescence: suitable immunoprecipitation (IP): suitable western blot: suitable |
input | sample type induced pluripotent stem cell(s) sample type: human embryonic stem cell(s) |
同位素/亚型 | IgM |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich