Anti-TRIM3 Antibody, clone 13D12.1

产品说明

一般描述

Tripartite motif-containing protein 3 (UniProt O75382; also known as Brain-expressed RING finger protein, RING finger protein 22, Ring finger protein 97, Tripartite motif protein TRIM3, Tripartite motif-containing 3) is encoded by the TRIM3 (also known as BERP, HAC1, RNF22, RNF97) gene (Gene ID 10612) in human. TRIM3 belongs to a family of more than 70 TRIM/RBCC proteins characterized by their shared tripartite motif structure, also known as the RBCC (RING finger, B-box, and coiled-coil) domain. The B-box and coiled coil regions are believed to participate in protein-protein interactions and formation of macromolecular complexes. TRIM proteins are involved in diverse cellular processes, including cell proliferation, differentiation, oncogenesis, and apoptosis. The presence of a RING domain suggests that TRIM proteins function as E3 ubiquitin ligases. TRIM3 is reported to catalyze the transfer of ubiquitin from UbcH5a to p21, a protein necessary for the proliferation of a subset of PDGF (platelet-derived growth factor)-transformed proneural glioma cells. Loss of TRIM3 heterozygosity is found in more than 10% of human GBMs (glioblastomas), while reducing TRIM3 expression facilitates tumor development and accelerates the progression of oligodendroglioma in a mouse model.

免疫原

GST-tagged recombinant protein corresponding to the internal fragment of human TRIM3.

应用

Anti-TRIM3 Antibody, clone 13D12.1 is an antibody against TRIM3 Antibody for use in Western Blotting and Immunohistochemistry.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected TRIM3 in mouse brain and human skeletal muscle tissue.

质量

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TRIM3 in 10 µg of HEK293 cell lysate.

目标描述

~80 kDa observed. 80.8 kDa (Isoform 1), 79.6 kDa (Isoform 2), 72.1 kDa (Isoform 3), 67.7 kDa (Isoform 4) calculated.

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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