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Anti-U1 snRNP C (U1C) antibody, Rat monoclonal

品牌
Sigma-Aldrich
货号
SAB4200188
规格纯度
clone 4H12, purified from hybridoma cell culture
参考价格
5241.99 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-U1 snRNP C (U1C) antibody, Rat monoclonal
Anti-SNRPC
Anti-U1-C
Anti-Snrp1c
Anti-U1C
Anti-U1 small nuclear ribonucleoprotein C
产品介绍:

产品说明

一般描述

The U1 snRNP complex contains the U1 snRNA molecule and the U1 snRNP specific proteins U1-70K, U1A and U1C, plus a common set of eight proteins, called Sm proteins U1A and U1-70K contain RNA binding domains and interact with naked snRNA on their own.
Monoclonal Anti-U1 snRNP C (U1C) (rat IgG2a isotype) is derived from the hybridoma 4H12 produced by the fusion of mouse myeloma cells (SP2) and splenocytes from a rat immunized with mouse U1C protein. Small nuclear ribonucleoprotein polypeptide C (U1C) is mapped to human chromosome 6p21.31.

特异性

Monoclonal Anti-U1 snRNP C (U1C) recognizes human, monkey, mouse, rat, and hamster U1C.

免疫原

mouse U1C protein fusion protein

应用

Anti-U1 snRNP C (U1C) antibody, Rat monoclonal has been used for:

  • immunoblotting
  • immunofluorescence
  • immunoprecipitation
  • immunocytochemistry

生化/生理作用

The U1 small nuclear ribonucleoprotein particle (snRNP) has an important function in the early formation of the spliceosome, the multicomponent complex in which pre-mRNA splicing takes place. The binding of U1C to the U1snRNP particle is dependent on protein-protein interactions between U1C and U1-70K as well as U1C and the common Sm proteins.
U1A and U1−70K contain RNA binding domains and interact with naked snRNA on their own. In cultured HeLa cells, mutant U1C proteins that are not able to bind to the U1 snRNP do not accumulate in the nucleus, indicating that nuclear accumulation of U1C is due to incorporation of the protein into the U1 snRNP.

外形

Solution in 0.01M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

储存及稳定性

Store at -20 ℃. For continuous use, store at 2-8 ℃ for up to one month. For extended storage, freeze at -20 ℃ in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.41

产品性质

生物来源rat
偶联物unconjugated
抗体形式purified from hybridoma cell culture
antibody product typeprimary antibodies
克隆4H12, monoclonal
形式buffered aqueous solution
分子量 ~20 kDa
species reactivityhamster, rat, human, monkey, mouse
浓度~1.0 mg/mL
technique(s)immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using HeLa or COS7 or CHO cell extracts
同位素/亚型IgG2a
UniProt登记号P09234
运输dry ice
储存温度−20℃
Gene Informationhuman ... SNRPC(6631)
mouse ... Snrpc(20630)
rat ... LOC685273(685273)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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