产品说明
一般描述
Monoclonal Anti-USP8 (mouse IgG1 isotype) is derived from the hybridoma US872 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Ubiquitin specific protease 8 (USP8) belongs to the family of de-ubiquitinating enzymes (DUBs).
免疫原
synthetic peptide corresponding to an internal sequence of human USP8, conjugated to KLH.
应用
Monoclonal Anti-USP8 antibody produced in mouse has been used in:immunoblotting immunofluorescence,flow cytometry, immunocytochemistry.
生化/生理作用
Ubiquitin specific protease 8 (USP8) was found to regulate ubiquitination of frizzled, a receptor for Wnt/Wingless. Deletion of USP8 in mice causes embryonic lethality. The defect was accompanied by a strong reduction or absence of several growth factor receptor tyrosine kinases (RTKs), like EGFR, hepatocyte growth factor receptor (c-met), and ERBB3.USP8 inactivation results in enhanced ubiquitination of ligand-activated EGFR. USP8 in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect epidermal growth factor receptor (EGFR) as well as other receptors from lysosomal degradation.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | US872, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~127 kDa antigen ~70 kDa |
| species reactivity | monkey, dog, human, rat, bovine, mouse |
| 浓度 | ~1.0 mg/mL |
| technique(s) | flow cytometry: 5-10 μg/test using MCF7 cells indirect immunofluorescence: 1.25-2.5 μg/mL using A549 cells western blot: 0.5-1.0 μg/mL using MCF7 total cell extracts |
| UniProt登记号 | P40818 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... USP8(9101) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
